P9614
Lambda protein phosphatase
liquid, Bacteriophage Lambda, recombinant, expressed in E. coli
Sinónimos:
Lambda-PPase
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About This Item
Productos recomendados
recombinant
expressed in E. coli
Quality Level
form
liquid
mol wt
25 kDa
concentration
≥400000 units/mL
shipped in
dry ice
storage temp.
−70°C
General description
Lambda protein phosphatase is a recombinant protein expressed in Escherichia coli. It requires Mn2+ or Ni2+ as activators.
Biochem/physiol Actions
Lambda protein phosphatase has been used to dephosphorylate HeLa cell extracts.
Can be used to release phosphate groups from serine, threonine or tyrosine residues in proteins. Also active on phosphorylated histidine residues.
Unit Definition
One unit will hydrolyze 1 nanomole of p-nitrophenyl phosphate per min at pH 7.5 at 30 deg C.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
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