P4380

Protamine sulfate

powder

• Sinónimos: Salmine, Salmine from salmon
• Número de CAS: 53597-25-4
• MDL number: MFCD00132091
• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Protamine sulfate salt from salmon, Grade II
• biological source: fish (salmon)
• Quality Level: 200
• type: Grade II
• form: powder
• color: white to off-white
• solubility: 6 M HCl: 25 mg/mL, clear, colorless to light yellow

Descripción

General description

Protamine sulfate (PS), a polycationic, positively charged protein[1] is mostly made up of poly(l-arginine).[2] It is obtained from salmon sperm protein.[1]

Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine sulfate salt from salmon has been used in the generation of protamine silica nanocomposites.[3]

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Protamine sulfate is highly preferred in cardiovascular surgical procedures to reverse the anticoagulant properties of heparin.[4] It is used for biomedical and biotechnological applications due to its biodegradablity.[2]

Quality

May contain insolubles as well as histones.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)