This product is also soluble in water at 10mg/ml. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/195/258/p4020pis.pdf
P4020
Protamine sulfate
amorphous powder
Sinónimos:
Salmine, Salmine from salmon
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1 G
US$ 75,70
5 G
US$ 133,00
10 G
US$ 300,00
25 G
US$ 1.120,00
100 G
US$ 2.030,00
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1 G
US$ 75,70
5 G
US$ 133,00
10 G
US$ 300,00
25 G
US$ 1.120,00
100 G
US$ 2.030,00
About This Item
Productos recomendados
Nombre del producto
Protamine sulfate salt from salmon, Grade X, amorphous powder
type
Grade X
Quality Level
form
amorphous powder
impurities
histone, free (Millon test)
color
white to off-white
solubility
6 M HCl: 25 mg/mL
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General description
Application
May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Biochem/physiol Actions
Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Los clientes también vieron
Steve N Bailey et al.
Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin
Diazeniumdiolation of protamine sulfate reverses mitogenic effects on smooth muscle cells and fibroblasts
Van Lith R, et al.
Free radical biology & medicine, 82, 13-21 (2015)
Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
The design and evaluation of heparin-binding foldamers.
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PloS one, 5(3), e9463-e9463 (2010-03-09)
Podocytes are injured in several glomerular diseases. To alter gene expression specifically in podocytes in vivo, we took advantage of their active endocytotic machinery and developed a method for the targeted delivery of small interfering ribonucleic acids (siRNA). We generated
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Are there other solvents besides 6M HCl to bring protamine sulfate salt into solution?
1 answer-
Helpful?
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Bonjour, Pourriez vous me confirmer qu'il s'agit bien d'un polypeptide et de combien d'unité de monomère est il formé ? Pourriez vous m'envoyer la représentation de la molécule ? merci
1 answer-
Unfortunately, structural studies on for this product have not been performed. However, regarding structure characterization and molecular representation of protamine from salmon, the following publication may be of interest for reference.
J Biol Chem. 1959 Sep:234:2314-6.
Physical and chemical properties of protamine from the sperm of salmon (Oncorhynchus tschawytscha). II. Anion binding characteristics
https://pubmed.ncbi.nlm.nih.gov/13807973/Helpful?
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