M1028

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

• Sinónimos: MgCl₂, dichloromagnesium, magnesium chloride
• Fórmula lineal: MgCl₂
• Número de CAS: 7786-30-3
• Peso molecular: 95.21
• MDL number: MFCD00011106
• UNSPSC Code: 12352302
• PubChem Substance ID: 24896582
• NACRES: NA.52
• grade: for molecular biology
• form: liquid
• solubility: water: soluble

Propiedades

• grade: for molecular biology
• Quality Level: 300
• sterility: non-sterile; 0.2 μm filtered
• form: liquid
• concentration: 1.00 M±0.01 M
• technique(s): DNA sequencing: suitable
• impurities: DNAse, none detected; NICKase, none detected; Protease, none detected; RNAse, none detected
• solubility: water: soluble
• density: 1.080 g/cm³
• cation traces: heavy metals (as Pb): ≤5 ppm
• suitability: suitable for electrophoresis; suitable for molecular biology
• application(s): cell analysis
• foreign activity: DNase, RNase, NICKase and protease, none detected
• SMILES string: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• InChI key: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Descripción

General description

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -

• to prepare buffer to lyse cells for immunoprecipitation[1]
• as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.[2]
• in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.[3]
• It has been used to protect the liver from oxidative damage.[4]
• to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.[5]

Features and Benefits

• Available as a Ready-to-use solution which reduces the need for preparation time.
• Heavy metals (as Pb): ≤5 ppm
• Free from DNase, RNase, NICKase and protease

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Other Notes

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Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves