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Merck

L7395

Sigma-Aldrich

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

Sinónimos:

15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase

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15000000 UNITS
US$ 265,00
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US$ 1.570,00

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15000000 UNITS
US$ 265,00
75000000 UNITS
US$ 905,00
150000000 UNITS
US$ 1.570,00

About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

US$ 265,00

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tpo

Type I-B

Formulario

lyophilized powder

actividad específica

≥50,000 units/mg solid

mol peso

94 kDa

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Descripción general

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.[1]

Aplicación

Lipoxidase from Glycine max (soybean) has been used:
  • to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins[2]
  • as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives[3]
  • as a positive control in lipoxygenase inhibitory activity assay[4]

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Acciones bioquímicas o fisiológicas

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases.[5] These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.[1]

Definición de unidad

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Forma física

Contains stabilizer and NaCl

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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