It is Laminin-1.
L4544
Human Laminin
from human fibroblasts, cell culture derived, liquid, 0.5 mg/mL, suitable for cell culture
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nombre del producto
Laminin from human fibroblasts, cell culture derived, liquid, sterile-filtered
origen biológico
human fibroblasts
Nivel de calidad
esterilidad
sterile-filtered
Formulario
liquid
envase
pkg of 100 μL
técnicas
cell culture | mammalian: suitable
cobertura de la superficie
1‑2 μg/cm2
Nº de acceso UniProt
Especificidad de unión
Peptide Source: Collagen
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... LAMB1(3912)
Aplicación
Laminin from human fibroblasts has been used:
Laminin from human fibroblasts is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
- in coating six-well plates for wound healing assay[1]
- as a substrate in cell adhesion and spreading assay[2]
- for differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) toward dopaminergic neurons[3]
- in corneal endothelial cell wound healing (migration) assay and corneal endothelial cell barrier assay[4]
Laminin from human fibroblasts is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Acciones bioquímicas o fisiológicas
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Componentes
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is produced by human fibroblasts and epithelial cells in a co-culture system and then purified biochemically.
Precaución
It is recommended to store this product at -70°C, where it will remain stable for two years.
Nota de preparación
This product is supplied at a concentration of 0.5 mg/mL in TBS. Thaw this solution slowly before use at 2-8°C to avoid gel formation. If at any time prior to that, the laminin is allowed to warm up prematurely, gelling can occur. Moreover, even allowing it to be stored at -20C can induce gelling or significantly reduce its activity or both." For use as a coating, dilute in a HBSS, coat culture surface with a minimal volume and incubate at 37°C for 1-2 hours. Wash 3 times with HBBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
Lukovic D, et al.
Stem Cells Translational Medicine, 6(4), 1217-1226 (2017)
The depletion of MARVELD1 leads to murine placenta accreta via integrin I?4-dependent trophoblast cell invasion.
Chen, et al.
Journal of Cellular Physiology, 233, 2257-2269 (2017)
Elucidating the molecular basis of PPCD: Effects of decreased ZEB1 expression on corneal endothelial cell function
Zakharevich M, et al.
Molecular Vision, 740-740 (2017)
Serum-induced differentiation of glioblastoma neurospheres leads to enhanced migration/invasion capacity that is associated with increased MMP9
Joseph JV, et al
PLoS ONE, 10(12), e0145393-e0145393 (2015)
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Artículos
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Protocolos
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
-
Hi! Do you know what is the predominant laminin isoform in the preparation? Thank you!
1 answer-
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
