L-15 Medium (Leibovitz) is formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate supplements. It supports established cell lines, such as human larynx epidermoid cells (HEp-2) and Rhesus monkey kidney epithelial cells (LLC-MK2), as well as primary explants of embryonic and adult human tissue. L-15 medium can also be used for culturing viruses.
Application
L-15 Medium (Leibovitz) has been used:
to study the toxicity of silver nanoparticles (AgNPs) with primary cultures of Eisenia fetida coelomocytes[1]
to culture crab hepatopancreas and ovary tissues[2]
as a component of the dissociation medium for infusion of the pancreas of mice[3]
Quantity
Formulated to contain 13.8 grams of powder per liter of medium.
Ranavirus is the second most common infectious cause of amphibian mortality. These viruses affect caudates, an order in which information regarding Ranavirus pathogenesis is scarce. In the Netherlands, two strains (CMTV-NL I and III) were suspected to possess distinct pathogenicity
Insect biochemistry and molecular biology, 27(5), 387-395 (1997-05-01)
Prostaglandins of the 2-series (e.g. PGE2) are typically synthesized from arachidonic acid (AA) after AA is released from cellular phospholipids after activation of an intracellular phospholipase A2 (PLA2). Treatment of isolated salivary glands with PLA2 inhibitor oleyloxyethyl phosphorylcholine (OPC) or
Effect of estradiol on vitellogenesis and oocyte development of female swimming crab, Portunus trituberculatus
Genotoxicity assessment is of high relevance for crude and refined petroleum products, since oil compounds are known to cause DNA damage with severe consequences for aquatic biota as demonstrated in long-term monitoring studies. This study aimed at the optimization and
In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline
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