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Merck

M1018

Sigma-Aldrich

Medio esencial mínimo Eagle

With Hanks′ salts, ʟ-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medio esencial mínimo Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Quantity

Formulated to contain 10.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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