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Merck

H6533

Sigma-Aldrich

Anti-HA monoclonal

clone HA-7, purified from hybridoma cell culture

Sinónimos:

Anti-HA, Hemaglutinina anti-gripal

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.56
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biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent

storage temp.

2-8°C

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General description

Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA).[1] HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.[2]
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Specificity

El anticuerpo reconoce tanto las formas nativas como las desnaturalizadas-reducidas de las proteínas etiquetadas con HA y es reactivo con las proteínas de fusión etiquetadas con HA en N-terminal o C-terminal expresadas en E. coli o en células de mamífero.

Immunogen

péptido sintético correspondiente a un fragmento de la hemaglutinina (HA) del virus de la gripe humana, conocida como etiqueta-HA, conjugado con KLH

Application

Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
  • in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells[3]
  • in immunoblot detection in transfected A375 cells[4]
  • is suitable for immunoblotting of nucleosome assembly protein NAP1L1[5]

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Reconstitute in .5mL of distilled water.

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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Hepatitis C virus NS5A targets nucleosome assembly protein NAP1L1 to control the innate cellular response
Cevik RE, et al.
Journal of virology, 91(18), e00880-e00817 (2017)
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Luo M, et al.
Cellular Signalling, 42, 30-43 (2018)
Ernest C So et al.
The Journal of biological chemistry, 291(11), 5832-5843 (2016-01-13)
Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the
Paulina Bartuzi et al.
Nature communications, 7, 10961-10961 (2016-03-12)
The low-density lipoprotein receptor (LDLR) plays a pivotal role in clearing atherogenic circulating low-density lipoprotein (LDL) cholesterol. Here we show that the COMMD/CCDC22/CCDC93 (CCC) and the Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) complexes are both crucial for endosomal sorting
A broadly reactive human anti-hemagglutinin stem monoclonal antibody that inhibits influenza a virus particle release
Yamayoshi S, et al.
EBioMedicine, 17, 182-191 (2017)

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    1 answer
    1. Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

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