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GE17-6003-18

DeStreak Reagent

Cytiva 17-6003-18

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About This Item

EC Number:
UNSPSC Code:
41105323
NACRES:
NA.25

form

liquid

feature

wetted part: no

packaging

pkg of 1 mL

manufacturer/tradename

Cytiva 17-6003-18

storage condition

(Rehydration Solution -20)

color

Colorless

storage temp.

2-8°C

Categorías relacionadas

General description

DeStreak Reagent.

Application

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.

DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak Reagent is also available separately in a 1-ml pack size.

Features and Benefits

  • Improves reproducibility and quality of 2-D gels by preventing streaking.
  • Eliminates extra spots caused by nonspecific oxidation of Proteins.
  • Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
  • Highly suitable for both analytical and preparative gels.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

DeStreak is a trademark of Cytiva

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Skull and crossbones

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Danger

hcodes

Hazard Classifications

Acute Tox. 3 Oral - Eye Dam. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

233.6 °F - closed cup

flash_point_c

112 °C - closed cup


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Protocolos

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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