This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
D8662
Disolución salina tamponada con fosfatos de Dulbecco
Dulbecco’s Phosphate Buffered Saline
2 of 3 reviewers received a sample product or took part in a promotion
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Sinónimos:
DPBS
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nombre del producto
Disolución salina tamponada con fosfatos de Dulbecco, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR[1]
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells[2]
- to wash cells for protein isolation[2]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
related product
Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Anna Eskilsson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(19), 5035-5044 (2017-04-26)
Fever occurs upon binding of prostaglandin E
L Parmegiani et al.
Journal of assisted reproduction and genetics, 35(10), 1887-1895 (2018-08-04)
To study the efficacy and efficiency of a "universal warming protocol" for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP). Two studies on patients undergoing fertility treatments via ICSI: a prospective randomized
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
Protocolos
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
-
What is the concentration of MgCl2 and CaCl2?
1 answer-
Helpful?
-
-
¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
