A5968

Anti-AP-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Ap-1 Antibody, Ap1 Antibody, Ap1 Antibody - Anti-AP-1 antibody produced in rabbit, Anti-c-Jun
• Número MDL: MFCD02095845
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 39 kDa
• reactividad de especies: human
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human colon carcinoma; microarray: suitable; western blot: 1:200 using HeLa nuclear extract
• Nº de acceso UniProt: P05412
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... JUN(3725)

Descripción

Descripción general

Activation Protein transcription factor 1 (AP-1) is a leucine zipper transcription factor that recognizes a specific palindromic sequence present in several promoters and enhancers. AP-1 can be induced by several growth and apoptosis-related signaling pathways. Jun proteins are also involved in metastatic, inflammatory and stress responses.
Anti-AP-1/c-Jun recognizes an epitope located on the c-Jun DNA binding domain. This epitope is highly conserved in c-Jun, Jun B and Jun D proteins of chicken, mouse, rat, and human. By immunoblotting, the antibody reacts specifically with AP-1/c-Jun (a single band or occasionally a doublet at 39kDa region). Additional bands of lower molecular weight may be observed. Staining of AP-1/c-Jun band(s) is inhibited by the AP-1/c-Jun immunizing peptide (amino acid residues 246-263).

Inmunógeno

synthetic peptide corresponding to amino acids 246-263 of human c-Jun with N-terminally added lysine, conjugated to KLH.

Aplicación

Anti-AP-1 antibody has been used in

• immunohistochemistry[1]
• immunochemistry
• western blotting[1]

Immunofluorescence of Hela cells was performed using monoclonal anti-AP1 (clone 100/3) as the primary antibody.

Acciones bioquímicas o fisiológicas

Activation Protein transcription factor 1 (AP-1) recognizes a specific sequence (TPA response element, a palindromic TRE sequence TGA(C/G) TCA or related sequences) present in many promoter and enhancer gene regions. Regulation of AP-1 is achieved by changes in the expression of Jun and Fos proteins in the dimer, by phosphorylation at both Ser⁶³ and Ser⁷³ by c-Jun N-terminal kinases (JNK) and by interaction with a variety of transcriptional coactivators like C-Jun activation domain-binding protein-1 (JAB1). AP-1 is induced by a variety of signals including those eliciting proliferation, differentiation and apoptosis.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable