11772457001
Roche
TUNEL AP
sufficient for 70 tests, solution, pkg of 3.5 mL
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About This Item
Código UNSPSC:
41105600
Productos recomendados
Formulario
solution
uso
sufficient for 70 tests
envase
pkg of 3.5 mL
fabricante / nombre comercial
Roche
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
TUNEL AP is an alkaline phosphatase-labeled antibody used for the in situ detection of apoptosis (programmed cell death) with the TUNEL reaction followed by transmission light microscopy.
The tailing reaction using TdT, also named ISEL (in situ end labeling) or TUNEL (TdT-mediated dUTP nick end labeling), has several advantages in comparison to the in situ nick translation (ISNT) using DNA polymerase:
- Label intensity of apoptotic cells is higher with TUNEL compared to ISNT, resulting in an increased sensitivity.
- Kinetics of nucleotide incorporation is very rapid with TUNEL compared to the ISNT.
- TUNEL preferentially labels apoptotic cells compared to necrotic cells.
Aplicación
TUNEL AP is an antibody that is used to convert fluorescence-based TUNEL assays into colorimetric assays suited for light microscopy. The conversion is performed by binding of an anti-fluorescein antibody to FITC-dUTP. The antibody is labeled with alkaline phosphatase (AP). The AP is visualized with a precipitating substrate, such as Fast Red or NBT/BCIP.
Componentes
Anti-fluorescein antibody, Fab fragment from sheep, conjugated with alkaline phosphatase (AP). Ready-to-use solution.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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