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MAB5310

Sigma-Aldrich

Anti-Glutathione Antibody, clone D8

clone D8, Chemicon®, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

D8, monoclonal

reactividad de especies

eukaryotes

fabricante / nombre comercial

Chemicon®

técnicas

western blot: suitable

isotipo

IgG2a

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Especificidad

Reacts with reduced or non-reduced glutathione on proteins only. Does not react with free glutathione.

Inmunógeno

Glutathione-protein complexes.

Aplicación

Anti-Glutathione Antibody, clone D8 is an antibody against Glutathione for use in WB.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western blotting of glutatione conjugated proteins under non-reduced conditions: 1:500-1:1000; does not react with glutatione by itself.

Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)

Optimal working dilutions must be determined by end user.

Forma física

Format: Purified
Immunoglobulin purified by protein A affinity and presented as a liquid in PBS, pH 7.2 with 0.01% sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.
Klaus, A; Zorman, S; Berthier, A; Polge, C; Ramirez, S; Michelland, S; Seve, M; Vertommen et al.
Testing null
Tatiana A Seregina et al.
International journal of molecular sciences, 24(22) (2023-11-25)
Inactivation of enzymes responsible for biosynthesis of the cell wall component of ADP-glycero-manno-heptose causes the development of oxidative stress and sensitivity of bacteria to antibiotics of a hydrophobic nature. The metabolic precursor of ADP-heptose is sedoheptulose-7-phosphate (S7P), an intermediate of
Vladimir Alexandrovich Mitkevich et al.
Frontiers in pharmacology, 10, 922-922 (2019-09-03)
Exogenous RNases are selectively toxic to tumor cells. The reasons for this selectivity are not quite clear and should be searched for in the properties that distinguish malignant from normal cells. During onco-transformation, cells acquire properties allowing them to adapt
Marina K Kukhanova et al.
Oxidative medicine and cellular longevity, 2019, 3196140-3196140 (2019-11-07)
Hepatitis C virus (HCV) triggers massive production of reactive oxygen species (ROS) and affects expression of genes encoding ROS-scavenging enzymes. Multiple lines of evidence show that levels of ROS production contribute to the development of various virus-associated pathologies. However, investigation
Yugo Tsuchiya et al.
The Biochemical journal, 474(14), 2489-2508 (2017-03-28)
Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved in major metabolic pathways, allosteric interactions and the regulation of gene expression. Abnormal biosynthesis and homeostasis of CoA and its derivatives have

Questions

  1. Hi, we would like to see if this antibody works for drosophila samples. Is it possible to order a small samples?  In addition, does adding SDS in the protein preparation interrupt the use of this antibody? Thanks, Liping

    1 answer
    1. The species reactivity of this antibody is Eukaryote; it is expected to react with Drosophila. The Western blotting analysis of glutathione conjugated proteins was performed under non-reduced conditions. Interference in the presence of sodium dodecyl sulfate has not been investigated. Note that this antibody does not react with free glutathione.

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