We conducted tests on 4 NHP samples and were able to detect EGF, Albumin, A1M, Clusterin, and Cystatin C in the samples. However, we were not able to detect OPN or NGAL, although the absence of signal could be attributed to low levels in the few samples tested. It's important to note that the kit has not been tested with NHP standards to determine the extent of reactivity.
HKI2MAG-99K
MILLIPLEX® Human Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay
The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).
Sinónimos:
Human Kidney Toxicity Multiplex Assay, Luminex® Human Kidney Toxicity Assay, Millipore Human Kidney Toxicity Panel
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
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Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 95-109%
sensitivity: 0.015-5.359 ng/mL
(MinDC = 2SD)
standard curve range: 0.01-10 ng/mL
(EGF)
standard curve range: 0.02-20 ng/mL
(Lipocalin-2/ NGAL)
standard curve range: 0.20-200 ng/mL
(Cystatin C)
standard curve range: 0.59-600 ng/mL
(Osteopontin (OPN))
standard curve range: 0.98-1,000 ng/mL
(α-1-Microglobulin)
standard curve range: 3.91-4,000 ng/mL
(Albumin)
standard curve range: 4.88-5,000 ng/mL
(Clusterin)
inter-assay cv: <20%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
Maintenance of physiological balance in the body is attributed in part to normal kidney function. Altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. Toxicity is the leading cause of drug failure, so research is expanding in search of more sensitive, rapid methods to determine organ-specific damage as quickly as possible. Traditionally, blood urea nitrogen (BUN) and serum creatinine tests have been used to determine drug-induced damage to the kidney. These tests only detect kidney damage after it begins and do not allow for the elucidation of where in the kidney the damage has occurred. Specificity and earlier detection of kidney injury is vital, and this area of research is expanding in search of more sensitive, rapid methods for determining the specific area of damage in kidney.
The MILLIPLEX® portfolio provides valuable research assays to investigate multiple biomarkers of kidney injury in human urine samples using the Luminex® xMAP® instrument platform. The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).
Panel Type: Toxicity
The MILLIPLEX® portfolio provides valuable research assays to investigate multiple biomarkers of kidney injury in human urine samples using the Luminex® xMAP® instrument platform. The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).
Panel Type: Toxicity
Specificity
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Application
- Analytes: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN)
- Recommended Sample type: Urine
- Recommended Sample dilution: 1:100 in kit Assay Buffer. Customers need to determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
- Assay Run Time: Overnight
- Research Category: Toxicity
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Other Notes
Sensitivity: Please see kit protocol for individual assay sensitivities.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Irene Capelli et al.
In vivo (Athens, Greece), 34(3), 1333-1339 (2020-05-02)
Acute kidney injury is an important cause of mortality in very-low-birth-weight (VLBW) preterm infants. As in the general population, the detection of renal damage cannot rely on the measurement of serum creatinine, since it has been demonstrated to be a
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Do any of the analytes available in the Milliplex Human Kidney Injury Panel 2 exhibit cross-reactivity with NHP samples?
1 answer-
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How should urine samples for use in the Milliplex Kidney Injury Panels be stored? Is it better to store them at 4°C if they will be used in a few days or go through a freeze/thaw cycle?
1 answer-
The samples remain stable for up to one day at 4°C, but for longer-term storage, it is preferable to store them at -80°C. Additionally, the samples are stable for up to one freeze/thaw cycle. If more freeze/thaw cycles are required, it is best to aliquot the samples.
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