ABC74

Anti-IGPR-1 Antibody

1 mg/mL, from rabbit

• Sinónimos: Transmembrane and immunoglobulin domain-containing protein 2, Immunoglobulin and proline-rich receptor 1, IGPR-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rat, human
• concentración: 1 mg/mL
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001162597
• Nº de acceso UniProt: Q96BF3
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TMIGD2(126259)

Descripción

Descripción general

IGPR-1 or Immunoglobulin and proline-rich receptor 1, also called Transmembrane and immunoglobulin domain-containing protein 2 and encoded by the human gene TMIGD2/IGPR1 is a widely expressed epithelial and endothelial membrane protein that plays a role in cell-cell interactions, cell migration and angiogenesis. IGPR-1 regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. IGPR-1 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of IGPR-1 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that IGPR-1 regulates capillary tube formation in vitro, and in B16F melanoma cells engineered to express IGPR-1 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, IGPR-1, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)-containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2.

Inmunógeno

Epitope: Cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of the human mature form of IGPR-1.

Aplicación

Anti-IGPR-1 antibody is an antibody against IGPR-1 for use in western blotting, ICC & IHC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2 µg/mL from a representative lot detected IGPR-1 in 10 µg of HepG2 cell lysate, human placenta tissue lysate, HUVEC cell lysate, and L9 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected IGPR-1 in IGPR-1 transfected PAE cell lysates (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected IGPR-1 in IGPR-1 transfected PAE cells (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected IGPR-1 in human colon cancer tissues (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Calidad

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IGPR-1 in 10 µg of PC-12 cell lysate.

Descripción de destino

~58 kDa observed. Uniprot describes two isoforms at 31 kDa and 30 kDa The pre-mature and mature form of this protein may be observed at 32 kDa and 58 kDa (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.). Uncharacterized band(s) may be observed in some cell lysates.

Forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable