14-864
PAK-1 PBD Protein (agarose free), 300 µg
For use in Affinity Binding Assays, ELISA & G-Protein Assays.
Sinónimos:
p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
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About This Item
Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
human
Nivel de calidad
mol peso
Mw 21 kDa
fabricante / nombre comercial
Upstate®
concentración
>50% (Coomassie blue staining, SDS-PAGE)
2 mg/mL
técnicas
ELISA: suitable
affinity binding assay: suitable (G-protein)
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
Descripción general
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
Calidad
Routinely evaluated by G-protein activity assay
Forma física
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
Almacenamiento y estabilidad
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Nota de análisis
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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