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07-539

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys 4) Antibody

Upstate®, from rabbit

Sinónimos:

H3K4Ac, Histone H3 (acetyl K4)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human

fabricante / nombre comercial

Upstate®

técnicas

ChIP: suitable (ChIP-seq)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

acetylation (Lys 4)

Información sobre el gen

human ... H3F3B(3021)

Especificidad

Broad species cross reactivity is expected
Histone H3 when acetylated on Lys4

Inmunógeno

KLH-conjugated, synthetic peptide containing the sequence ...RT[AcK]Q... in which [AcK] corresponds to acetyl lysine 4 of human histone H3

Aplicación

Suggested dilutions:
Western Blot: 1:1000 - 1:10,000
Anti-acetyl-Histone H3 (Lys 4) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys 4) also known as H3K4Ac, Histone H3 (acetyl K4) & has been validated in Mplex, WB, PIA, ChIP, ChIP-seq, DB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Calidad

Routinely evaluated by immunoblot.

Descripción de destino

Mr ~17kDa

Forma física

100μl of immunoaffinity purified rabbit IgG in a buffer with 0.1M Tris-glycine, pH 7.4 and 0.15M NaCl, and containing 0.05% sodium azide and 30% glycerol
ImmunoAffinity Purified

Almacenamiento y estabilidad

1 year at -20°C from date of shipment

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1


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Federico Forneris et al.
The Journal of biological chemistry, 281(46), 35289-35295 (2006-09-22)
Human lysine-specific demethylase (LSD1) is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys4 of histone H3 (H3-K4). We used a combination of in vivo and in vitro experiments to characterize the substrate specificity and recognition
Ya-Wei Chen et al.
Cancer, 119(24), 4259-4267 (2013-12-05)
Patterns of global histone modifications have been suggested to be predictors of clinical outcome in many cancers. However, the role of global histone modification patterns in oral squamous cell carcinoma (OSCC) is unclear. A retrospective clinicopathologic analysis was undertaken of
Flore Beurton et al.
Nucleic acids research, 47(21), 11164-11180 (2019-10-12)
The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin
Oral administration of the pimelic diphenylamide HDAC inhibitor HDACi 4b is unsuitable for chronic inhibition of HDAC activity in the CNS in vivo.
Beconi, M; Aziz, O; Matthews, K; Moumne, L; O'Connell, C; Yates, D; Clifton, S; Pett et al.
Testing null
Runsheng He et al.
Nucleic acids research, 45(11), 6427-6441 (2017-04-13)
Epigenetic regulation of chromatin plays a critical role in controlling embryonic stem (ES) cell self-renewal and pluripotency. However, the roles of histone demethylases and activating histone modifications such as trimethylated histone 3 lysine 4 (H3K4me3) in transcriptional events such as

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