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T5569

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB substrate for ELISA (Super Slow)

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate

form

liquid

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

Application

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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