P5607

L-Proline

98.5-101.0% dry basis, suitable for cell culture, non-animal source, meets EP, USP testing specifications

• Synonym(s): (S)-Pyrrolidine-2-carboxylic acid
• Empirical Formula (Hill Notation): C₅H₉NO₂
• CAS Number: 147-85-3
• Molecular Weight: 115.13
• Beilstein: 80810
• EC Number: 205-702-2
• MDL number: MFCD00064318
• UNSPSC Code: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 24898677
• NACRES: NA.26

Properties

• Product Name: L-Proline, from non-animal source, meets EP, USP testing specifications, suitable for cell culture
• biological source: non-animal source
• Quality Level: 300
• Agency: meets EP testing specifications; meets USP testing specifications
• Assay: 98.5-101.0% dry basis
• form: powder
• optical activity: [α]20/D -86.0 to -84.0 °, c = 4 in water; [α]25/D -86.3 to -84.3°, c = 4 in water
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• mp: 228 °C (dec.) (lit.)
• solubility: H₂O: 50 mg/mL
• cation traces: Fe: ≤10 ppm; NH₄⁺: ≤0.02%
• application(s): pharmaceutical (small molecule)
• functional group: (Imine); carboxylic acid
• storage temp.: room temp
• SMILES string: OC(=O)[C@@H]1CCCN1
• InChI: 1S/C5H9NO2/c7-5(8)4-2-1-3-6-4/h4,6H,1-3H2,(H,7,8)/t4-/m0/s1
• InChI key: ONIBWKKTOPOVIA-BYPYZUCNSA-N

Description

General description

L-Proline, also known as 2-Pyrrolidinecarboxylic acid, is a versatile amino acid that plays an essential role in various biological processes. It is classified as a member of the glutamine family of amino acids and serves as a building block for proteins. L-Proline is vital for maintaining the structural integrity of proteins, including collagen, which is crucial for the proper functioning of joints, tendons, and various connective tissues.

This amino acid, synthesized within the human body from other amino acids, is involved in a range of functions. It contributes to wound healing, supports the synthesis of essential molecules such as elastin and carnitine, and is a key player in protein synthesis and structural stability. L-Proline is also associated with metabolic processes, including the synthesis of arginine, polyamines, and glutamate. In the realm of nutrition, it is known for its antioxidative properties and its role in immune responses.

Additionally, It also influences carbohydrate metabolism, cell growth, proliferation, and the maintenance of normal bodily functions. In cellular studies, it has demonstrated its ability to stimulate the growth of specific cell types while inhibiting the growth of others, making it a multifaceted component of various biological systems.

Moreover, L-Proline operates as a signaling molecule, participating in redox reactions and serving as a sensor of cellular energy status. It is involved in differentiation processes, impacting embryonic stem cells and the growth and development of the conceptus (fetus and associated extraembryonic membranes) in humans and animals[1]. L-Proline is a critical amino acid that plays a diverse and significant role in various biochemical and cellular processes, making it a valuable subject of study in fields such as biochemistry, microbiology, metabolism, cell biology, and nutrition research.

Application

L-proline has been used as a component of high-glucose-Dulbecco′s modified Eagle′s medium (HG-DMEM) for chondrocyte differentiation[2] and for the culture of chondrocyte-like cells.[3] It has also been used as a component of yeast nitrogen base for yeast cultures.[4]

Biochem/physiol Actions

Proline is a cyclic, non-essential, hydrophobic amino acid.[5] It is a proteinogenic amino acid which is crucial for primary metabolism.[6] In peptide chains, proline residues confer structural constraints and enhance the susceptibility of proximal peptide bonds to protease activity.[7]

Features and Benefits

• Can be used in Metabolomics and Biochemical research
• High-quality compound suitable for multiple research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves