This is a complete medium, no additional supplements are required. Please see the link below to review the complete protocol for the culture of Human Osteoblasts. This product is referenced in Section V. Differentiating HOB:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/primary-cell-culture/human-osteoblasts
417D-250
Human Osteoblast Differentiation Medium (250 ml)
Synonym(s):
Osteogenic medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.
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form
liquid
Quality Level
shelf life
6 mo.
manufacturer/tradename
Cell Applications, Inc
shipped in
wet ice
storage temp.
2-8°C
General description
Human Osteoblast Differentiation Medium
Application
Human Osteoblast Differentiation Medium (250 ml) has been used to induce differentiation in human primary osteoblasts.[1]
Quality
Each lot was tested for the ability to support plating, spreading and proliferation
Preparation Note
contained in protocol
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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FBS, 항생제, 분화에 필요한 ascorbic acid, b-glycerophosphate를 별도 첨가하지 않고 바로 사용해도 되나요.
1 answer-
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Buenas tardes, He comprado este medio para diferenciar mis células MSCs, y me gustaría saber cuales son los compuestos específicos que lo componen y en qué cantidad. Un saludo, Elvira
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The formulation of the medium is proprietary information.
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