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529576

Sigma-Aldrich

PP2

≥95% (HPLC), liquid, Src protein tyrosine kinase inhibitor, Calbiochem®

Synonym(s):

InSolution PP2

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN5
Molecular Weight:
301.77
UNSPSC Code:
12352200
NACRES:
NA.77

product name

PP2, InSolution, ≥95%, 10 mM, reversible ATP-competitive inhibitor of the Src family of protein tyrosine kinases

Quality Level

100

Assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

clear colorless

shipped in

wet ice

storage temp.

−20°C

General description

A potent and selective inhibitor of the src family of tyrosine kinases. Its action is similar to PP1. Selectively inhibits p56lck (IC50 = 4 nM), p59fynT (IC50 = 5 nM), and Hck (IC50 = 5 nM) compared to other tyrosine kinases, such as EGF-R (IC50 = 480 nM), JAK2 (IC50 >50 µM) or ZAP-70 (IC50 >100 µM). Also potently inhibits anti-CD3-stimulated tyrosine phosphorylation of human T cells (IC50 = 600 nM).

Biochem/physiol Actions

Cell permeable: no
Primary Target
p56lck
Product competes with ATP.
Reversible: yes
Target IC50: 4 nM, 5 nM, 5 nM, 100 nM, against p56lck, p59fynT, Hck , and Src, respectively; 600 nM against anti-CD3-stimulated tyrosine phosphorylation of human T cells

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (1 mg/331 µl) solution of PP2 (Cat. No. 529573) in DMSO.

Reconstitution

Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).

Other Notes

Hanke, J.H., et al. 1996. J. Biol. Chem.271, 695.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point(C)

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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