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G0376

Sigma-Aldrich

β-Glucuronidase from bovine liver

Type B-3, ≥2,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
Enzyme Commission number:
3.2.1.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine liver

Quality Level

200

type

Type B-3

form

solid

specific activity

≥2,000,000 units/g solid

storage temp.

−20°C

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Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Helena C Besse et al.
Pharmaceutics, 12(6) (2020-06-14)
The poor pharmacokinetics and selectivity of low-molecular-weight anticancer drugs contribute to the relatively low effectiveness of chemotherapy treatments. To improve the pharmacokinetics and selectivity of these treatments, the combination of a doxorubicin-glucuronide prodrug (DOX-propGA3) nanogel formulation and the liberation of
Hendrik Modick et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 925, 33-39 (2013-03-23)
N-Acetyl-4-aminophenol (NAAP) is the major urinary metabolite of aniline. The general population is known to be ubiquitously exposed to aniline through various sources. Furthermore, NAAP, known under the trade name paracetamol (resp. acetaminophen), is one of the most commonly used
Xiaoming Ye et al.
PloS one, 8(1), e55615-e55615 (2013-02-06)
βKlotho is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also
Ivana Hálová et al.
The Journal of biological chemistry, 288(14), 9801-9814 (2013-02-28)
Chemotaxis, a process leading to movement of cells toward increasing concentrations of chemoattractants, is essential, among others, for recruitment of mast cells within target tissues where they play an important role in innate and adaptive immunity. Chemotaxis is driven by
Yu Zhu et al.
Cancer science, 104(6), 663-671 (2013-02-26)
Klotho is an anti-aging protein predominantly expressed in renal tubular epithelial cells. Although Klotho was recently identified as a tumor suppressor gene in a variety of cancers, the potential role and molecular events for Klotho in renal cell carcinoma (RCC)
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Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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