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D3159

Sigma-Aldrich

Deoxyribonucleic acid from herring sperm

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp), degraded

Synonym(s):

Deoxyribonucleic acid, partially degraded from herring sperm, hsDNA

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About This Item

EC Number:
309-566-6
MDL number:
MFCD00130921
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

200

form

powder

suitability

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp)

storage temp.

2-8°C

General description

Herring Sperm DNA, also known as hsDNA is a natural source of DNA designed for DNA binding agent studies. (1) The detection limits of hsDNA are around 5.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-2.5 mµg.[1]

Application

Herring sperm DNA (hsDNA) is a natural DNA used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Herring sperm DNA is also used in physicochemical studies of DNA behavior in solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000375495
0000373580
0000360492
0000360493
0000375495

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Yanyan Niu et al.
Journal of chromatography. A, 1463, 102-109 (2016-08-16)
Fufang Banbianlian Injection (FBI) has been widely used as an anti-inflammatory and anti-tumor prescription. To understand the relationships between its bioactive ingredients and pharmacological efficacies, our previous study has been successfully identified some DNA-binding compounds in FBI using an established
Synthesis, spectroscopic, crystal structure and DNA binding of Ru(II) complexes with 2-hydroxy-benzoic acid [1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-ethylidene]-hydrazide.
Chitrapriya N, Sathiya Kamatchi T, et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 81, 128-134 (2011)
Yan Zhang et al.
Nucleosides, nucleotides & nucleic acids, 30(1), 49-62 (2011-01-25)
The Mg(II) complex of Schiff base (K[HL]) derived from vanillin and L-tryptophan could bind with herring sperm DNA. The binding behaviors between them in physiological pH environment (pH 7.40) have been studied by spectroscopy, cyclic voltammetry and viscosity methods. Binding
Po Wang et al.
Biosensors & bioelectronics, 26(7), 3339-3345 (2011-02-08)
A rapid, convenient and accurate method for the simultaneous detection of guanine (G), adenine (A), thymine (T) and cytosine (C) was developed at a multiwalled carbon nanotube (MWCNT)/choline (Ch) monolayer-modified glassy carbon electrode (GCE). X-ray photoelectron spectroscopy data demonstrated that
M N Patel et al.
Journal of enzyme inhibition and medicinal chemistry, 26(3), 359-366 (2011-01-29)
A series of ternary copper(II) complexes have been derived using levofloxacin and five phenanthroline derivatives. Complexes were characterized using infrared spectroscopy, Thermogravimetric (TG)-analysis, fast atom bombardment mass spectroscopy and reflectance spectra. Synthesized complexes exhibit the only d-d band at ∼
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Questions

1–5 of 5 Questions  

  1. Is it suitable for melting temperature analysis?

    1 answer

    1. This product has not been tested for melting temperature analysis. The product is not considered to be highly purified. This is an application that will have to be validated by the end user.

      It may be helpful to review the literature. See the links below for publications that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275468/
      https://pubmed.ncbi.nlm.nih.gov/9056205/
      https://www.researchgate.net/publication/313712083_Probing_the_Effects_on_the_Thermal_Stability_of_Salmon_Sperm_DNA_in_the_Presence_of_Metal_Cations_by_Measuring_DNA_Melting_Temperatures

      Helpful?

  2. How should I reconstitute Product D3159, Deoxyribonucleic acid?

    1 answer

    1. D3159 is soluble in water at 5 mg per mL. Add slowly to stirring water and then heat gently (60° C or less) or dissolve overnight at 4° C

      Helpful?

  3. How should I store Product D3159, Deoxyribonucleic acid?

    1 answer

    1. DNA has been stored successfully for several months at 4°C, pH 7.5 - 8, in 10 mM Tris with 1 mM EDTA. The addition of a bacteriostatic agent is not necessary.

      Helpful?

  4. What is the molecular weight range of Product D3159, Deoxyribonucleic acid?

    1 answer

    1. The molecular weight range is 10,000 - 30,000 Daltons. (One base pair is approximately 660 Daltons.)

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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