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D2001

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Type VIII

Synonym(s):

DNA

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

type

Type VIII

Quality Level

form

fibers

impurities

<2% protein

storage temp.

2-8°C

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Application

Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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O Schröder et al.
Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)
The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction
Joanna Will et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(3), 421-434 (2007-12-25)
Combined multidimensional liquid chromatography and electrospray ionisation tandem mass spectrometry was employed to analyse platinated tryptic peptides from Escherichia coli cells treated with the anticancer drug cis-[PtCl2(NH3)2] at pH 7.0. Prerequisites for the LC/LC/MS/MS analysis of protein targets that are
Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli
Hiller M, et al.
Chembiochem, 6(9), 1679-1684 (2005)
Geoffrey S Briggs et al.
The Journal of biological chemistry, 282(17), 12353-12357 (2007-02-20)
The DNA-binding protein, RdgC, is associated with recombination and replication fork repair in Escherichia coli and with the virulence-associated, pilin antigenic variation mediated by RecA and other recombination proteins in Neisseria species. We solved the structure of the E. coli
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In

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