Yes, yeasts are eukaryotic organisms. α-Amanitin inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase.
A2263
α-Amanitin
From Amanita phalloides, ≥85% (HPLC), RNA polymerase II and III inhibitor, powder
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About This Item
Empirical Formula (Hill Notation):
C39H54N10O14S
CAS Number:
Molecular Weight:
918.97
UNSPSC Code:
12352202
NACRES:
NA.32
PubChem Substance ID:
EC Number:
245-432-2
MDL number:
Beilstein/REAXYS Number:
1071138
Assay:
≥85% (HPLC)
Form:
powder
Pricing and availability is not currently available.
Product Name
α-Amanitin, from Amanita phalloides, ≥85% (HPLC), powder
biological source
Amanita phalloides
Quality Segment
assay
≥85% (HPLC)
form
powder
mol wt
918.97 g/mol
color
white to light yellow
mp
254-255 °C (lit.)
solubility
H2O: 1.0 mg/mL
ε (extinction coefficient)
13,500 at 310 nm in H2O at 1 M
storage temp.
−20°C
SMILES string
CCC(C)C1NC(=O)CNC(=O)C2Cc3c([nH]c4cc(O)ccc34)S(=O)CC(NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)N5CC(O)CC5C(=O)NC(C(C)C(O)CO)C(=O)N2
InChI
1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)
InChI key
CIORWBWIBBPXCG-UHFFFAOYSA-N
Application
α-Amanitin has been used:
- for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay
- to induce nephrotoxicity in mice renal tissues
- to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay
Biochem/physiol Actions
Inhibits eukaryotic RNA polymerase II and III; does not inhibit RNA polymerase I or bacterial RNA polymerase; inhibits mammalian protein synthesis.
The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.
Preparation Note
Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.
Disclaimer
When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years.
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥85% (HPLC) | assay ≥85% (HPLC) | assay ≥85% (HPLC) | assay ≥85% (HPLC) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 918.97 g/mol | mol wt - | mol wt - | mol wt - |
| solubility H2O: 1.0 mg/mL | solubility - | solubility water: 50 mg/mL, clear, colorless to faintly yellow | solubility H2O: 50 mg/mL |
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Oral - STOT RE 2
target_organs
Kidney,Blood,Liver,Gastrointestinal tract,Respiratory Tract,Central nervous system
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A2263-100MG | 04061833228029 |
| A2263-1MG | 04061833349946 |
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Can we use this product (alpha amanitin: A2263) to block RNA PII for yeast cell (S. cerevisiae)?
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