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G8295

β-Glucuronidase from Escherichia coli

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC Number:
Specific activity:
≥10,000,000 units/g protein (30 min assay)
Recombinant:
expressed in E. coli overproducing strain
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recombinant

expressed in E. coli overproducing strain

Quality Segment

form

lyophilized powder

specific activity

≥10,000,000 units/g protein (30 min assay)

mol wt

~290 kDa

composition

protein, 40-60% biuret

storage temp.

−20°C

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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1 of 1

This Item
G7646G0799G0751
specific activity

≥10,000,000 units/g protein (30 min assay)

specific activity

5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

specific activity

>20,000,000 units/g protein

specific activity

≥300,000 units/g solid

form

lyophilized powder

form

lyophilized powder

form

aqueous glycerol solution

form

partially purified powder

recombinant

expressed in E. coli overproducing strain

recombinant

-

recombinant

expressed in E. coli

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

~290 kDa

mol wt

290 kDa

mol wt

74 kDa

mol wt

-

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200


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pictograms

Health hazard

signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1



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