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β-Glucuronidase from Escherichia coli

Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00199013

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

>20,000,000 units/g protein

Recombinant:

expressed in E. coli

Concentration:

>1 mg/mL

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Properties

recombinant

expressed in E. coli

Quality Segment

200

form

aqueous glycerol solution

specific activity

>20,000,000 units/g protein

mol wt

74 kDa

concentration

>1 mg/mL

shipped in

wet ice

storage temp.

−20°C

Description

General description

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

Application

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

New Technical Article Comparing Performance of Different Enzymes
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about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Highly purified solution in 50% glycerol

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

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This Item	G8162	G8295	G7396
Sigma-Aldrich G0799 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G8162 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G8295 β-Glucuronidase from Escherichia coli	Sigma-Aldrich G7396 β-Glucuronidase from Escherichia coli
description >20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution	description aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)	description ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder	description Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
specific activity >20,000,000 units/g protein	specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret)	specific activity ≥10,000,000 units/g protein (30 min assay)	specific activity 1,000,000-5,000,000 units/g protein (30 min assay)
concentration >1 mg/mL	concentration -	concentration -	concentration -
recombinant expressed in E. coli	recombinant -	recombinant expressed in E. coli overproducing strain	recombinant -
form aqueous glycerol solution	form aqueous glycerol solution	form lyophilized powder	form lyophilized powder
shipped in wet ice	shipped in wet ice	shipped in -	shipped in wet ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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