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Esterase from porcine liver

Name: Esterase from porcine liver
Brand: SIGMA

lyophilized, powder, slightly beige, ≥50 U/mg

Synonym(s):

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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About This Item

CAS Number:

9016-18-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-773-7

MDL number:

MFCD00131030

EC Number:

3.1.1.1 (BRENDA, IUBMB)

Specific activity:

≥50 U/mg

Biological source:

Porcine liver

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Properties

biological source

Porcine liver

Quality Segment

100

form

lyophilized solid, powder

quality

lyophilized

specific activity

≥50 U/mg

mol wt

M_r ~162000

color

slightly beige

storage temp.

−20°C

Description

General description

Porcine liver esterase (PLE) is localized in the endoplasmic reticulum (ER).

Application

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Esterase from porcine liver has been used as a negative control in fluorescence measurement studies.

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Biochem/physiol Actions

Porcine liver esterase (PLE) displays good stability, broad substrate specificity, and is a low-cost enzyme. It is useful in the hydrolysis of ester- and amide-containing compounds, to free acids and is useful detoxification of xenobiotics.

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol ethyl valerate (CAt. No. 30784) per minute at pH 8.0 and 25°C

contains 1,4-dithioerythritol

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This Item	E3019	E0887	43388
Sigma-Aldrich 46058 Esterase from porcine liver	Sigma-Aldrich E3019 Esterase from porcine liver	Sigma-Aldrich E0887 Esterase from rabbit liver	Sigma-Aldrich 43388 Esterase Isoenzyme 1 porcine liver, recombinant
description lyophilized, powder, slightly beige, ≥50 U/mg	description lyophilized powder, ≥15 units/mg solid	description lyophilized powder, ≥30 units/mg protein	description recombinant, expressed in E. coli, ≥30.0 U/g
specific activity ≥50 U/mg	specific activity ≥15 units/mg solid	specific activity ≥30 units/mg protein	specific activity ≥30.0 U/g
biological source Porcine liver	biological source -	biological source -	biological source -
form lyophilized solid, powder	form lyophilized powder	form lyophilized powder	form crystalline, crystals, powder or flakes
mol wt M_r ~162000	mol wt 168 kDa	mol wt 60 kDa	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 100	Quality Level 300	Quality Level 200	Quality Level 100

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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