Skip to Content
Merck
All Photos(3)

Documents

N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonym(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
4264-83-9
Molecular Weight:
263.05
MDL number:
MFCD00007319
UNSPSC Code:
12352204
PubChem Substance ID:
24897835
NACRES:
NA.83

product name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

form

liquid

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Caution

Stable at least one year when stored below 0 °C.

Physical form

Ready-to-use.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Target Organs

Kidney,Liver,Blood

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

I K Manoylov et al.
Clinical and experimental immunology, 197(3), 329-340 (2019-04-23)
Type 1 diabetes mellitus is an autoimmune metabolic disorder characterized by chronic hyperglycemia, the presence of autoreactive T and B cells and autoantibodies against self-antigens. A membrane-bound enzyme on the pancreatic beta-cells, glutamic acid decarboxylase 65 (GAD65), is one of
J V Jones et al.
Journal of immunological methods, 118(1), 79-84 (1989-03-10)
We have recently described a substrate amplification system, based on the method of Self, which increases the sensitivity of alkaline phosphatase (AP)-dependent enzyme-linked immunosorbent assays (ELISA) by a factor of 30-50. This increase is achieved by having AP, the primary
Humanization of anti-human insulin receptor antibody for drug targeting across the human blood-brain barrier
Boado RJ, et al.
Biotechnology and Bioengineering, 96(2), 381-391 (2007)
Evaluation of selective laser sintered polyamide/hydroxyapatite composite compositions-in vitro and in vivo
Kamarajan, et al.
International journal of biomedical research, 80(8), 467-474 (2007)
Peter Van den Eede et al.
PloS one, 6(1), e16257-e16257 (2011-02-08)
There is an increasing body of literature reporting treatment failure of the currently recommended radical treatment of Plasmodium vivax infections. As P. vivax is the main malaria species outside the African continent, emerging tolerance to its radical treatment regime could
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service