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F8630

Sigma-Aldrich

Fibrinogen from bovine plasma

Type I-S, 65-85% protein (≥75% of protein is clottable)

Synonym(s):

Factor I

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About This Item

CAS Number:
9001-32-5
EC Number:
232-598-6
MDL number:
MFCD00163624
UNSPSC Code:
12352202
NACRES:
NA.61
Pricing and availability is not currently available.

biological source

bovine plasma

type

Type I-S

form

powder

mol wt

α-chain 63.5 kDa
β-chain 56 kDa
γ chain 47 kDa (about 4% carbohydrate content)
soluble dimer 340 kDa

concentration

65-85% (biuret)

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02672
P02676
P12799

storage temp.

−20°C

Gene Information

cow ... FGA(522039) , FGB(510522) , FGG(280792)

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Application

Fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP).

Biochem/physiol Actions

Acute phase protein that is part of the coagulation cascade of proteins.
Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyzes fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Physical form

Contains ~10% sodium citrate and ~15% sodium chloride.

Reconstitution

The optimal way to solubilize fibrinogen is to layer it on top of warm (37 ºC) saline, as fibrinogen will not dissolve in water. The saline concentration can be in the range of 0.85-0.9%. The fibrinogen-saline solution can be gently agitated, but it must not be vortexed. The fibrinogen will slowly dissolve to give a hazy solution. Fibrinogen may be sterile-filtered, but may not go through a 0.1 μm filter. A 0.2 μm filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to breakdown of the molecule during the filtration.

Analysis Note

Protein determined by biuret

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000431933
0000431929
0000431929
0000431933
0000420137

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Katarzyna Kubiak et al.
Colloids and surfaces. B, Biointerfaces, 139, 123-131 (2015-12-27)
Adsorption kinetics of fibrinogen at a gold substrate at various pHs was thoroughly studied using the QCM-D method. The experimental were interpreted in terms of theoretical calculations performed according to the random sequential adsorption model (RSA). In this way, the
Sunghoon Lee et al.
Nature nanotechnology, 14(2), 156-160 (2019-01-02)
In biointegrated electronics, the facile control of mechanical properties such as softness and stretchability in electronic devices is necessary to minimize the perturbation of motions inherent in biological systems1-5. For in vitro studies, multielectrode-embedded dishes6-8 and other rigid devices9-12 have
Navid Hakimi et al.
Lab on a chip, 18(10), 1440-1451 (2018-04-18)
We present a handheld skin printer that enables the in situ formation of biomaterial and skin tissue sheets of different homogeneous and architected compositions. When manually positioned above a target surface, the compact instrument (weight <0.8 kg) conformally deposits a
C Fenger-Eriksen et al.
British journal of anaesthesia, 101(6), 769-773 (2008-09-27)
Patients experiencing massive haemorrhage are at high risk of developing coagulopathy through loss, consumption, and dilution of coagulation factors and platelets. It has been reported that plasma fibrinogen concentrations may reach a critical low level relatively early during bleeding, calling
Johanna Prüller et al.
PloS one, 13(9), e0202574-e0202574 (2018-09-18)
Biophysical/biochemical cues from the environment contribute to regulation of the regenerative capacity of resident skeletal muscle stem cells called satellites cells. This can be observed in vitro, where muscle cell behaviour is influenced by the particular culture substrates and whether
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Is it ok to use PBS to reconstitute? PBS has 0.9% saline. After reconstitution can I freeze/thaw?

    1 answer

    1. Quality control has tested dissolving this product using 0.9% NaCl. Customer feedback indicates that some lots may precipitate when using PBS for dissolution. The optimal way to solubilize fibrinogen is to layer it on top of warm (37 °C) saline. Fibrinogen does not dissolve in water. The saline concentration can be in the range of 0.85-0.9%. The fibrinogen-saline solution can be gently agitated but must not be vortexed. Fibrinogen will slowly dissolve to give a hazy solution. Fibrinogen may be sterile-filtered, but may not go through a 0.1 µm filter. A 0.2 µm filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to a breakdown of the molecule during filtration.

      After sterile filtration, stock solutions of fibrinogen at 2.5 mg/ml may be stored refrigerated for about 1 week.

      Helpful?

  2. Il prodotto (fibrinogeno F8630-5g) viene venduto sterile? Grazie!

    1 answer

    1. This Fibrinogen product is not sterile. Currently, a sterile version of this material is not available. As noted in the 'DESCRIPTION' section, under 'Reconstitution, Fibrinogen may be sterile-filtered, but may not go through a 0.1 μm filter. A 0.2 μm filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to the breakdown of the molecule during the filtration.

      Helpful?

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