581616-U
Ascentis® Phenyl (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
Phenyl HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Ascentis® Phenyl HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L11
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
19% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
450 m2/g
surface coverage
5.2 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
phenyl phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
Application
- A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison: This study highlights the advanced chromatographic techniques used to characterize pharmaceutical compounds, demonstrating the importance of accurate and detailed analysis in pharmaceutical research. The use of the Ascentis® Phenyl HPLC column facilitates high-resolution separations, essential for identifying degradation products in complex mixtures (Geven et al., 2023).
- HPLC with Spectrophotometric or Mass Spectrometric Detection for Quantifying Very-Long Chain Fatty Acids in Human Plasma and Its Association with Cardiac Risk Factors: This paper discusses the role of high-performance liquid chromatography (HPLC) in clinical settings, particularly for the assessment of biomarkers related to heart disease. Techniques involving the Ascentis® Phenyl HPLC column are employed to achieve precise and reliable quantification of fatty acids, critical for clinical diagnostics (Shrestha et al., 2021).
- A Fully Automated and Fast Method Using Direct Sample Injection Combined with Fused-Core Column On-Line SPE-HPLC for Determination of Ochratoxin A and Citrinin in Lager Beers: This study introduces an automated method for toxin analysis in beverages, utilizing the Ascentis® Phenyl HPLC column. The approach enhances sensitivity and throughput, showcasing the column′s efficacy in food safety applications (Lhotská et al., 2016).
- A Study of Retention Characteristics and Quality Control of Nutraceuticals Containing Resveratrol and Polydatin Using Fused-Core Column Chromatography: The research focuses on nutraceuticals, where the Ascentis® Phenyl HPLC column′s selectivity plays a pivotal role in segregating and analyzing health-promoting compounds, thereby ensuring product quality and compliance with industry standards (Fibigr et al., 2016).
- An On-Line SPE-HPLC Method for Effective Sample Preconcentration and Determination of Fenoxycarb and Cis, Trans-Permethrin in Surface Waters: This publication illustrates the application of the Ascentis® Phenyl HPLC column in environmental analysis, particularly for the sensitive detection of pesticides in water bodies. The column′s capabilities help in tracing low-level contaminants, critical for environmental monitoring and protection (Šatínský et al., 2015).
Features and Benefits
- 100% Aqueous Compatible
- ANP/HILIC and reversed-phase
- Low UV/MS bleed for gradient applications
- Alternate selectivity
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
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