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MAK112

Sigma-Aldrich

Arginase Activity Assay Kit

sufficient for 200 colorimetric tests

Synonym(s):

Arginase Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; immunological diseases; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ARG1(383) , ARG2(384)
mouse ... ARG1(11846) , ARG2(11847)
rat ... ARG2(29215) , Arg1(29221)

General description

Arginase is a manganese-containing enzyme that catalyzes the conversion of arginine to urea and ornithine.[1] Two isoforms of arginase are present in most mammals which differ in their tissue distribution and subcellular localization. Arginase I is a cytoplasmic protein predominantly expressed in the liver[2][3] where it catalyzes the fifth and final step of the Urea Cycle. Arginase II is a mitochondrial protein with broader tissue distribution whose function may include nitric oxide and polyamine metabolism. Decreased activity or expression of Arginase I results in the autosomal recessive disorder hyperarginemia.[2][3] Increased serum Arginase activity is implicated in hepatic injury and in certain pathological conditions such as cancer. Arginase also participates in multiple inflammatory reactions in the immune system, such as macrophage-mediated cytotoxicity.[1]

Application

Arginase Activity Assay Kit has been used to assess the activity of arginase.[4][5][6]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of arginase activity in enzyme preparations, serum, plasma, tissue culture, and similar samples. Suitable for studying the effects of drugs on arginase activity.

Principle

In this assay, arginase catalyzes the conversion of arginine to urea and ornithine. The urea produced specifically reacts with the substrate to generate a colored product, proportional to the arginase activity present.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H314,H360FD,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1A

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
112CE06A26
112CE06A19
112CE06A26
112CE06A19
112CD10A10

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Razieh Tavakoli Oliaee et al.
Microbial pathogenesis, 126, 368-378 (2018-11-07)
Detection of the mechanism of host/parasite interactions in unresponsive forms of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica is helpful for immunotherapy and vaccine development. In the present study, the gene expression of toll-like receptors (TLRs), TNF-α, iNOS and
Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency.
Martina H.
Journal of inherited metabolic disease, 39(3), 331?340-331?340 (2016)
Arginase activity, urea, and hydroxyproline concentration are reduced in keratoconus keratocytes.
Stachon T, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 255(1), 91-97 (2017)
Yuxin Shu et al.
Immunology, 160(4), 345-356 (2020-04-21)
Macrophages are particularly abundant and play an important role throughout the tumor progression process, namely, tumor-associated macrophages (TAM) in the tumor microenvironment. TAM can be polarized to disparate functional phenotypes, the M1 and M2 macrophages. M1-like type macrophages are defined
Andrew F Cumpstey et al.
Nitric oxide : biology and chemistry, 94, 27-35 (2019-10-12)
Native highlanders (e.g. Sherpa) demonstrate remarkable hypoxic tolerance, possibly secondary to higher levels of circulating nitric oxide (NO) and increased microcirculatory blood flow. As part of the Xtreme Alps study (a randomised placebo-controlled trial of dietary nitrate supplementation under field
View All Related Papers

Questions

1–3 of 3 Questions  

  1. Is an enzyme activation step of 55°C for 10 mins recommended if lysis was carried out at 4°C? If so, would the sample be mixed with the Mn solution first, followed by the arginine buffer after those 10 mins?

    1 answer

    1. The technical bulletin for this kit indicates to bring all reagents to room temperature prior to the assay and to preheat the arginine buffer to 37°C. The 5X substrate buffer is prepared by mixing arginine buffer with Mn solution in the described volumes shown in table 1. The assay reaction is performed by adding 10 uL of the previously prepared 5X substrate buffer to each of the sample wells. The incubation is performed at 2 hours at 37°C. Please review the technical bulletin for specific instructions on performing this assay.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/314/808/mak112bul.pdf

      There is no indication to heat the enzyme to 55°C or to mix your sample individually with Mn solution followed by arginine buffer.

      Helpful?

  2. Is this kit suitable to work on nonhepatic (kidney, specifically) tissue derived from chickens?

    1 answer

    1. This product has not been specifically tested for use in chicken kidney. However, it has been used successfully in a number of tissue types, including lung, neural, adipose, and tumor. This kit has also been successfully used with HEK 293T cells, which originate from embryonic kidney. Suitability would have to be determined by the end user. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/314/808/mak112bul.pdf

      Helpful?

  3. I would like to know whether this kit can be used with recombinant proteins and whether you recommend a specific one.

    1 answer

    1. This kit detects and measures the arginase activity, enzymatically. There are no anticipated problems detecting recombinant arginase, provided that the protein is enzymatically active.

      Helpful?

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