23479
Nitrite/Nitrate Assay Kit, colorimetric
used for detection of nitric oxide metabolites
Synonym(s):
Nitrate/Nitrite Assay Kit, colorimetric
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
![Nitrate Reductase (NAD[P]H) from Aspergillus niger lyophilized powder, ≥300 units/g solid](/deepweb/assets/sigmaaldrich/product/images/309/282/2a67ae4d-ca55-4f0b-96ec-34748ff8a21e/640/2a67ae4d-ca55-4f0b-96ec-34748ff8a21e.jpg)
![Nitrate Reductase (NAD[P]H) From Pichia Pastoris, recombinant freeze-dried protein glass](/deepweb/assets/sigmaaldrich/product/images/859/572/ae8d80df-c6d0-4cce-a526-35f8e21ca729/640/ae8d80df-c6d0-4cce-a526-35f8e21ca729.jpg)
usage
sufficient for 100 assays
suitability
passes test for application
storage temp.
2-8°C
Related Categories
Application
Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.
The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.
The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.
Kit Components Only
Product No.
Description
- NaNO2 Standard Solution (100 mM) 1 bottle
- NaNO3 Standard Solution (100 mM) 1 bottle
- Buffer solution (20 mM, pH 7.6) 1 bottle
- Nitrate reductase (lyophilized) 1 bottle
- Enzyme Co-factor (lyophilized) 1 bottle
- Griess Reagent A 1 bottle
- Griess Reagent B 1 bottle
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Junyao Yang et al.
Experimental cell research, 363(2), 262-270 (2018-01-22)
The X-box binding protein 1 (XBP1) is a pivotal transcription factor in the endoplasmic reticulum stress response. Our previous studies have proven that XBP1 is involved in vascular endothelial growth factor (VEGF)-mediated endothelial cell (EC) proliferation and angiogenesis. In this
Rong Wang et al.
Scientific reports, 6, 36790-36790 (2016-11-16)
Atherosclerosis is a serious public health concern. Excessive inflammatory responses of vascular cells are considered a pivotal pathogenesis mechanism underlying atherosclerosis development. It is known that Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signalling plays an important role
Othman A Al-Shboul et al.
Journal of pregnancy, 2019, 4302309-4302309 (2019-05-14)
It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the
Jolanta Zuwała-Jagiełło et al.
Clinical and experimental hepatology, 1(3), 105-111 (2015-11-01)
To determine plausible associations between liver cirrhosis and circulating endothelial cell-derived microparticles (EMPs), vascular endothelial growth factor (VEGF) levels and plasma nitric oxide (NO) metabolites. Sixty patients with cirrhosis and 20 healthy control subjects were enrolled in the study. Circulating
Othman A Al-Shboul et al.
Biomedical reports, 9(6), 511-516 (2018-12-14)
Previous studies have shown that progesterone could inhibit muscle contraction in various sites of the gastrointestinal tract. The underlying mechanisms responsible for these inhibitory effects of progesterone are not fully known. The aim of the current study was to investigate
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service