11082132001

Phytohemagglutinin-M (PHA-M)

from Phaseolus vulgaris

• Synonym(s): PHA-M
• UNSPSC Code: 23201100

Properties

• Quality Level: 100
• sterility: non-sterile; 0.2 μm filtered
• form: lyophilized
• packaging: pkg of 20 mg
• manufacturer/tradename: Roche
• application(s): hematology
• storage temp.: 2-8°C

Description

General description

Phytohemagglutinin (PHA), the lectin extract from the red kidney bean. PHA consists of five isolectins (L₄E₀, L₃E₁, L₂E₂, L₁E₃, L₀E₄) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.[1]

Application

Phytohemagglutinin (PHA) is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures.[2]

Phytohemagglutinin-M (PHA-M) has been used in the activation of Jurkat and primary cells.[3] It has also been used to treat polymorphonuclear blood cells(PMBCs)[4]

Biochem/physiol Actions

Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.[1]

Specifications

Biological activity: <10 μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes.

Preparation Note

Working concentration: 2 - 10 μg/ml
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.

Reconstitution

PHA-M should be reconstituted in sterile double-dist. water (final concentration: 2–10 mg/ml). Further dilution with medium or PBS (phosphate buffered saline).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash