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69238

Sigma-Aldrich

Rat Genomic DNA

High molecular weight, high purity DNA for any application

Synonym(s):

Rat DNA, Rat genomic DNA

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100 μG
$325.00

$325.00


Estimated to ship onMay 18, 2025



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100 μG
$325.00

About This Item

UNSPSC Code:
12352208
NACRES:
NA.51

$325.00


Estimated to ship onMay 18, 2025


Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

General description

Novagen genomic DNA is a purified preparation of high molecular weight genomic DNA obtained from various organisms. During the isolation process, measures are taken to minimize shearing and eliminate contaminants that could disrupt enzymatic procedures. It has been qualified for genomic analysis, including library construction and PCR, as at least 90% of the DNA is greater than 100 kbp in size, with contour-clamped homogeneous electric field (CHEF) electrophoresis analysis showing sizes greater than 50 kbp for S. cerevisiae. Notably, no inhibition of restriction enzyme activity has been observed, and the DNA is free from protein and RNA contaminants.

Application

Ratgenomic DNA has been used as a template for the amplification of theprogression elevated gene-3 (PEG-3) gene using polymerase chain reaction (PCR).[1]

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Regarding cat no. 69238 Rat Genomic DNA, which extraction method was used to extract the HMW DNA? We are specifically interested in rat mitochondrial DNA, is this still included?

    1 answer
    1. The isolation/extraction method for Rat Genomic DNA is proprietary and unavailable. The DNA is obtained from multiple animals from a single strain (Sprague Dawley) and a mixed sex population. The isolation protocol minimizes shearing and removes contaminants that inhibit enzymatic procedures. The DNA's origin, whether a mixture of mitochondrial and nuclear, solely mitochondrial, or nuclear, is not evaluated as part of the quality control process.

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