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GenomePlex® Complete Whole Genome Amplification (WGA) Kit

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

Synonym(s):

Whole genome amplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

GenomePlex® Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It contains everything required for whole genome amplification including an optimized enzyme, WGA DNA Polymerase. It can be used in several applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and serum. It allows the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:
  • microarray analysis
  • SNP analysis
  • STR analysis
  • DNA archiving

Features and Benefits

  • Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
  • Nanograms of samples can be preserved at –20 °C for future use
  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
  • Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
  • Whole-genome representation with no detectable allele bias
  • Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a
Tao Chen et al.
Toxicology, 292(2-3), 63-70 (2011-11-15)
Furan, a widely used industrial compound, has been found in a number of heated food items. Furan is carcinogenic to rats and mice, but the mechanism behind its carcinogenic effect is still not well understood. In this study, we tested
M Korabecna et al.
Neoplasma, 63(3), 402-410 (2016-03-02)
Tubulocystic renal cell carcinoma (TRCC) represents a rare tumor with incidence lower than 1 % of all renal carcinomas. This study was undertaken to contribute to characterization of molecular signatures associated with TRCC and to compare them with the features of papillary
Jinglan Jin et al.
Journal of cellular and molecular medicine, 23(11), 7474-7489 (2019-10-01)
We aimed to identify key genes and pathways associated with different immune statuses of hepatitis B virus (HBV) infection. The gene expression and DNA methylation profiles were analysed in different immune statuses of HBV infection. Differentially expressed genes (DEGs) and
Darius Juskevicius et al.
Laboratory investigation; a journal of technical methods and pathology, 98(11), 1487-1499 (2018-08-09)
Approximately 15% of patients with classical Hodgkin lymphoma (cHL) die after relapse or progressive disease. Comprehensive genetic characterization is required to better understand its molecular pathology and improve management. However, genetic information on cHL is hard to obtain mainly due
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Articles

Array CGH Analysis with Whole Genome Amplification

aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.

Array CGH Analysis of Challenging Samples

aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.

Whole Genome Amplification for Single Cell Biology

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

Multiplex PCR Assay for Damaged DNA

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

Protocols

DNA Extraction & WGA Amplification from Soil Samples

GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.

Saliva DNA Extraction & WGA Amplification

Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

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