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F4799

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

Synonym(s):

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

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About This Item

Empirical Formula (Hill Notation):
C120H169N31O49S
Molecular Weight:
2861.87
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD01863911
Pricing and availability is not currently available.
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Product Name

3X FLAG® Peptide, lyophilized powder

assay

≥90% (HPLC/MS)

Quality Level

200

form

lyophilized powder

shipped in

wet ice

storage temp.

2-8°C

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This Item
SAE0194P1306F3290
3X FLAG® Peptide lyophilized powder

Sigma-Aldrich

F4799

3X FLAG® Peptide

Purified 3xFLAG® peptide ≥95% (HPLC), lyophilized powder

Sigma-Aldrich

SAE0194

Purified 3xFLAG® peptide

Peptide YY human ≥97% (HPLC), synthetic

Sigma-Aldrich

P1306

Peptide YY human

FLAG® Peptide lyophilized powder

Millipore

F3290

FLAG® Peptide

assay

≥90% (HPLC/MS)

assay

≥95% (HPLC)

assay

≥97% (HPLC)

assay

≥85% (HPLC)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

solid

form

lyophilized powder

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.
The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG® affinity gels.


Learn more product details in our FLAG® application portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000469512
0000469513
0000469512
0000469513
0000339717

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Andrew T Schiffmacher et al.
The Journal of cell biology, 215(5), 735-747 (2016-11-20)
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest cells reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, to permit their EMT and migration. Serial processing of Cad6B by a
Min Hwa Shin et al.
PLoS genetics, 12(2), e1005884-e1005884 (2016-03-02)
The inactivation of p53 creates a major challenge for inducing apoptosis in cancer cells. An attractive strategy is to identify and subsequently target the survival signals in p53 defective cancer cells. Here we uncover a RUNX2-mediated survival signal in p53
Oriane Mauger et al.
Nucleic acids research, 43(3), 1869-1882 (2015-01-22)
Alternative splicing is the main source of proteome diversity. Here, we have investigated how alternative splicing affects the function of two human histone methyltransferases (HMTase): G9A and SUV39H2. We show that exon 10 in G9A and exon 3 in SUV39H2
Paul L Colbert et al.
Oncotarget, 6(2), 953-968 (2014-12-02)
Microtubules (MTs) are components of the cytoskeleton made up of polymerized alpha and beta tubulin dimers. MT structure and function must be maintained throughout the cell cycle to ensure proper execution of mitosis and cellular homeostasis. The protein tyrosine phosphatase
Tetsuya Hirata et al.
Nature communications, 9(1), 405-405 (2018-01-28)
Many eukaryotic proteins are anchored to the cell surface via the glycolipid glycosylphosphatidylinositol (GPI). Mammalian GPIs have a conserved core but exhibit diverse N-acetylgalactosamine (GalNAc) modifications, which are added via a yet unresolved process. Here we identify the Golgi-resident GPI-GalNAc
View All Related Papers

Articles

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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Global Trade Item Number

SKUGTIN
F4799-1MG04061824807516
F4799-4MG04061833617908
F4799-25MG04061833617892

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