306-05F
Human Cardiac Fibroblasts: HCF, fetal
Synonym(e):
Cardiac Fibroblasts, Fetal Cardiac Fibroblasts, HCF Cardiac Fibroblasts
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UNSPSC-Code:
41106514
NACRES:
NA.81
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Unterstützung erhaltenBiologische Quelle
human heart (normal)
Qualitätsniveau
Verpackung
pkg of 500,000 cells
Hersteller/Markenname
Cell Applications, Inc
Wachstumsmodus
Adherent
Karyotyp
2n = 46
Morphologie
fibroblast
Methode(n)
cell culture | mammalian: suitable
Relevante Krankheit(en)
cardiovascular diseases
Versandbedingung
dry ice
Lagertemp.
−196°C
Allgemeine Beschreibung
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Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).
Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:
- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
- Show that in response to mechanical stretch, cardiac fibroblasts release TGF-β which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
- Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (D’Souza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKα1 (Noppe, 2014) in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).
Anwendung
human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines
Biochem./physiol. Wirkung
Heart
Angaben zur Herstellung
- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Please refer to the HCF Culture Protocol.
Sonstige Hinweise
Basal Medium containing 10% FBS & 10% DMSO
Haftungsausschluss
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Protokolle
Culturing HCFs from human heart ventricles involves critical steps for studying cardiac-related conditions.
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