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biological source
human skeletal muscle (normal limb)
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 46
morphology
Skeletal muscle
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
muscular dystrophy
shipped in
dry ice
storage temp.
−196°C
General description
Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization to demonstrate AMPK signaling and responsiveness to insulin stimulation.
Cell Line Origin
Application
Components
Preparation Note
- 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture Routine
Disclaimer
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation
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