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C2432

Chloroform

contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Synonym(s):

Methylidyne trichloride, Trichloromethane

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25 mL

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$57.50
500 mL

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$96.60
1 L

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$119.00
4 x 25 mL
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$197.00
2.5 L

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$201.00
6 x 500 mL
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$499.00

About This Item

Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
UNSPSC Code:
12191502
NACRES:
NA.04
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
Assay:
≥99.5%
Technique(s):
RNA extraction: suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)

$57.50


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vapor density

4.1 (vs air)

Quality Level

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

contains

100-200 ppm amylenes as stabilizer

technique(s)

RNA extraction: suitable

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

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This Item
480150C7559366919
technique(s)

RNA extraction: suitable

technique(s)

-

technique(s)

PCR: suitable

technique(s)

UV/Vis spectroscopy: suitable

assay

≥99.5%

assay

≥99.8%

assay

≥99%

assay

≥99.8%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. The product contains amylene as a stabilizer, since chloroform is susceptible to degradation with time. Amylene acts as a scavenger of free radicals formed during chloroform decomposition.
Our biotech solvents offer exceptional quality for reliable RNA extraction, ensuring optimal laboratory performance. With low water content and minimal residue, give clean UV spectra to minimize contamination.
  • High-Quality Biotech Solvents: Designed for optimal laboratory performance with low water content and minimal residues.
  • Ideal for RNA Extraction: Essential for genetic testing and research applications like PCR.
  • Ensures Integrity of Genetic Material: Promotes reliable and reproducible results.
  • Industry Compliant: Suitable for both academic and commercial laboratories, enhancing research efficiency.

Application

Chloroform has been used for the isolation of RNA from mammalian tissue.[1]

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash


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Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells.
Ramos P et al.
Respiratory Research, 15, 54-54 (2014)
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species.
Kosma DK, et al.
Bio-protocol, 5(24) (2015)
RNA Isolation from Synechocystis.
Mironov KS and. Los DA.
Bio-protocol, 5(6) (2015)
Immune activation and target organ damage are consequences of hydrodynamic treatment but not delivery of naked siRNAs in mice.
Racz Z et al.
Nucleic Acid Therapeutics, 21, 215-215 (2011)
H Yamasaki et al.
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 322(2-3), 151-159 (1999-04-10)
Gap junctional intercellular communication (GJIC) is considered to play a key role in the maintenance of tissue independence and homeostasis in multicellular organisms by controlling the growth of GJIC-connected cells. Gap junction channels are composed of connexin molecules and, so

Articles

Se dice que las sustancias son miscibles entre sí si se disuelven hasta formar una disolución uniforme. Marque o descargue nuestra tabla de miscibilidad para los disolventes comunes del laboratorio.

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

Protocols

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Related Content

Product Information Sheet

Global Trade Item Number

SKUGTIN
C2432-6X500ML04061838254955
C2432-4X25ML04061838254931
C2432-25ML04061838123817
C2432-1L04061838254917
C2432-500ML04061838254948
C2432-2.5L04061838254924

Questions

1–8 of 8 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. I would like to know the water content of #C2432, can you tell me?

    1 answer
    1. This product is not tested on a lot-to-lot basis for moisture content and is not considered to be an anhydrous material. Historical data indicates water contents of less than 1%, however this material is not packaged under controlled conditions. Please see the link below to review the anhydrous product option:
      https://www.sigmaaldrich.com/product/sial/372978

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Has Product C2432, Chloroform, been tested for DNase and RNase activity?

    1 answer
    1. This product has not been tested for DNase and RNase activity. However, it has been tested and found to be suitable for the extraction of nucleic acids based upon the evaluation of extracted DNA by agarose electrophoresis.

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  5. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  6. What amount of amylenes are present in Product C2432, Chloroform, as a stabilizer?

    1 answer
    1. The concentration of amylenes is 100 - 300 ppm.

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  7. How can Product C2432, Chloroform, be used to extract proteins from nucleic acids?

    1 answer
    1. A protocol for using phenol:chloroform or phenol:chloroform:isoamyl alcohol to extract proteins from nucleic acids is described in Molecular Cloning: A Laboratory Manual, vol. 3, pp E.3 - E.4 (1989).

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  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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