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131164

Sigma-Aldrich

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

Synonym(s):

Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

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About This Item

Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
860-22-0
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein/REAXYS Number:
4103904
EC Number:
212-728-8
MDL number:
MFCD00005723
UNSPSC Code:
12171500
PubChem Substance ID:
24847997
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

assay

>85.0% (HPLC)

form

solid

composition

Dye content, 85%

color

dark blue to very dark blue

pH

3.87 (30 °C, 7.14 g/L)

mp

≥340 °C

density

0.71 g/cm3 at 29 °C

λmax

608 nm

εmax

20,000 at 250-256 nm in water at 0.01 g/L
29500 at 284-290 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR9009
SHBQ8945
SHBP6134
SHBN7381
SHBN1973

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Superoxide converts indigo carmine to isatin sulfonic acid: implications for the hypothesis that neutrophils produce ozone.
Kettle AJ
The Journal of Biological Chemistry, 279(18), 18521-18525 (2004)
An experimental study of indigo carmine removal from aqueous solution by electrocoagulation
Secula M S
Desalination, 277(1-3), 227-235 (2011)
J. Ind. Chem. Soc., 63, 533-533 (1986)
Rice husk ash as an effective adsorbent: evaluation of adsorptive characteristics for Indigo Carmine dye.
Lakshmi UR
Journal of Environmental Management, 90(2), 710-720 (2009)
Pancolonic indigo carmine dye spraying for the detection of dysplasia in ulcerative colitis
Rutter M D
Gut, 53(2), 256-260 (2004)
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