131164

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

• Synonym(s): Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
• Empirical Formula (Hill Notation): C₁₆H₈N₂Na₂O₈S₂
• CAS Number: 860-22-0
• Molecular Weight: 466.35
• Colour Index Number: 73015
• Beilstein/REAXYS Number: 4103904
• EC Number: 212-728-8
• MDL number: MFCD00005723
• UNSPSC Code: 12171500
• PubChem Substance ID: 24847997
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• assay: >85.0% (HPLC)
• form: solid
• composition: Dye content, 85%
• color: dark blue to very dark blue
• pH: 3.87 (30 °C, 7.14 g/L)
• mp: ≥340 °C
• density: 0.71 g/cm³ at 29 °C
• λmax: 608 nm
• εmax: 20,000 at 250-256 nm in water at 0.01 g/L; 29500 at 284-290 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O
• InChI: 1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;
• InChI key: KHLVKKOJDHCJMG-QDBORUFSSA-L

Description

Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Gloves