This product is packaged as a box of 8 tubes, 24 tubes, or 96 tubes.
UFC9030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
別名:
15 mL sample volume, 30 kDa MWCO, Centrifugal concentrator
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この商品について
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
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製品名
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO, sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL
15 mL
pore size
30 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
1 of 4
当該品目 | UFC8030 | UFC203024 | UFC5030 |
|---|---|---|---|
| parameter 15 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 0.5 mL sample volume |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 30 kDa MWCO | pore size 30 kDa MWCO | pore size 30 kDa MWCO | pore size 30 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。
溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
プロトコル
免疫グロブリンの精製プロトコルには通常、プロテインAまたはGクロマトグラフィー担体に対するアフィニティー結合、非結合タンパク質の洗浄、低pHバッファーによる溶出が含まれます。
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
関連コンテンツ
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
下流分析のための精製により、サンプルの複雑性を低減し、干渉を除去し、分析対象物質を濃縮することで、より正確な結果を得ることができます。
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how many units are there in 1 pkg
1 回答-
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How many individual columns come in one package. For example, if I wanted to purchase the 24package option, wil I get 24 columns or 24 packages of x number of columns?
1 回答-
A package of 24 would equate to 24 Ultra Centrifugal Filters.
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Hi there, I need to extract exosome in the range of 30 to 150 nm. Which Ultra Centrifugal Filter is fit for my work? Thank you for your advice in advance.
1 回答-
We have performed studies of nanometer particles and all of our studies show that using a 100 kda molecular weight cutoff should be fine to trap particles greater than 10 nm.
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Can this item be sterilised, and if so, what is the best method (e.g., autoclaving) to ensure that product function and integrity are maintained?
1 回答-
This item cannot be autoclaved. Sterilization with Ethylene Oxide is possible, but the method has not been validated.
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Can the product be washed and re-used?
1 回答-
This product is intended for single use. Repeated usage may result in declining performance.
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How many times can sample be added to the same filter unit? For example: If I have 100 mL cell free supernatant and want to add 12 mL at a time, centrifuge and collect retentate - followed by discarding the flow through and pouring the next 12 mL etc.
1 回答-
The same Amicon filter unit can be used for repeated spins with the same sample. However, repeated spins may lead to increased clogging, resulting in slower flow rates and longer spin times.
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I noticed no volume change after centrifuging 10mL cell media at 14,000xg for 20minutes. Could this be due to unsuccessful concentration?
1 回答-
The lack of volume change suggests that the sample may contain a high amount of particulate matter, leading to rapid clogging and preventing effective concentration.
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Hello! Our lab had to move to a different area of our building and we found some probably quite old boxes of Amicon Ultra 15s (Cat UFC903096, lot R8CN67048). Does this product have an expiration date/shelf life? If so, has this lot surpassed it?
1 回答-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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