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Sigma 7-9^®

Name: Sigma 7-9<SUP>&#174;</SUP>
Brand: SIGMA

≥99% (titration), crystalline, Sigma 7-9^®

Synonym(s):

Trizma^® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

NACRES:

NA.25

PubChem Substance ID:

24899961

UNSPSC Code:

12352104

EC Number:

201-064-4

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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Product Name

Sigma 7-9^®, ≥99% (titration), powder or crystals

description

aminopeptidase substrate

Quality Level

200

assay

≥99% (titration)

form

powder or crystals

storage condition

dry at room temperature

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable

impurities

≤0.5% water (Karl Fischer)

color

white to off-white

pH

10.5-12

Related Categories

Biochemicals

Biological Buffers

Tris

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1 of 1

This Item	T4661	T1503	T6791
Sigma-Aldrich T1378 Sigma 7-9^®	Sigma-Aldrich T4661 Trizma^® base	Sigma-Aldrich T1503 Trizma^® base	Sigma-Aldrich T6791 Trizma^® base
technique(s) ELISA: suitable, immunohistochemistry: suitable, flow cytometry: suitable	technique(s) -	technique(s) ELISA: suitable, protein extraction: suitable	technique(s) -
assay ≥99% (titration)	assay ≥99.9% (titration)	assay ≥99.9% (titration)	assay ≥99.9% (titration)
Quality Level 200	Quality Level 400	Quality Level 300	Quality Level 400
application(s) clinical research diagnostic assay manufacturing life science and biopharma sample preparation	application(s) diagnostic assay manufacturing	application(s) cell analysis diagnostic assay manufacturing life science and biopharma	application(s) diagnostic assay manufacturing life science and biopharma sample preparation
form powder or crystals	form crystalline	form crystalline	form crystalline
storage condition dry at room temperature	storage condition -	storage condition dry at room temperature	storage condition dry at room temperature

General description

Sigma 7-9^® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9^® has been used:

as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)[1]
as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence[2]

Features and Benefits

Suitable for Biological and Biochemical Research
Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

comparable product

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Description

Pricing

T6687

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T6791

Trizma^® base, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

T1503

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

T6066

Trizma^® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

T4661

Trizma^® base, ≥99.9% (titration), crystalline

93350

Trizma^® base, 99.7% (T), puriss. p.a.

93352

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93362

Trizma^® base, BioUltra, Molecular Biology, ≥99.8% (T)

33742

Trizma^® base, puriss. p.a., buffer substance, ≥99.5%

154563

Tris(hydroxymethyl)aminomethane, ultrapure grade, ≥99.9%

252859

Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

T1699

Trizma^® base solution, 1.5 M

RDD008

Trizma^® base, anhydrous, free-flowing, Redi-Dri^™, ≥99.9%

Storage Class

11 - Combustible Solids

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Global Trade Item Number

SKU	GTIN
T1378-500G	04061835266029
T1378-25KG	04061832552170
T1378-100G	04061835265992
T1378-100KG	04061832552156
T1378-10KG	04061837337291
T1378-50KG	04061832552187
T1378-1KG	04061835266005
T1378-5KG	04061837337321

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