33742

Trizma^® base

≥99.5%, crystalline, puriss p.a., aminopeptidase substrate

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein/REAXYS Number: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 329754644

Properties

• product name: Trizma^® base, puriss. p.a., buffer substance, ≥99.5%
• description: aminopeptidase substrate
• grade: puriss. p.a.
• assay: ≥99.5%
• form: crystalline
• impurities: ≤0.0005% heavy metals (as Pb); ≤0.5% water (Karl Fischer)
• ign. residue: ≤0.05% (as SO₄)
• color: white
• pH: 10.3-10.8 (0.1 M)
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.); 168-172 °C
• solubility: water: soluble (678 g/l at 20 °C)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Fe: ≤5 mg/kg
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves