Trypsin is active but autoproteolyze over time. It requires re-purification and design to reduce autoproteolysis.
T0303
Trypsin from porcine pancreas
Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
Trypsin from porcine pancreas
Synonym(s):
Serine Protease 1
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.78
EC Number:
232-650-8
MDL number:
Specific activity:
13,000-20,000 BAEE units/mg protein
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type
Type IX-S
Quality Level
form
lyophilized powder
specific activity
13,000-20,000 BAEE units/mg protein
mol wt
23.8 kDa
application(s)
diagnostic assay manufacturing
foreign activity
chymotrypsin ≤1.0 units/mg protein
storage temp.
−20°C
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| specific activity 13,000-20,000 BAEE units/mg protein | specific activity ≥6,000 BAEE units/mg protein | specific activity ≥9,000 BAEE units/mg protein | specific activity - |
| form lyophilized powder | form lyophilized powder | form essentially salt-free, lyophilized powder | form salt-free, lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt 23.8 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Biochem/physiol Actions
Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Preparation Note
This product is a lyophilized powder soluble in Hank′s Balanced Salt Solution at 25 mg/mL.
Other Notes
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. One BTEE unit = 320 ATEE units. Reaction volume = 3.2 mL (1 cm light path).
Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.
Disclaimer
Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocols
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T0303-1G | 04061835559190 |
| T0303-10G | 04061837334801 |
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