For saliva samples, P8340 is typically used at a 1:100 dilution (v:v), similar to its use in blood and tissue lysates. This means you would add 1 part of P8340 to 99 parts of saliva. However, the optimal concentration may need to be empirically determined for your specific application, as protease levels can vary between samples. There is no internal data on pre-diluting P8340 in water and storing it before use; the product is sold ready-to-use, and the best practice is to add it directly to your sample. If you wish to test pre-dilution and storage, you should validate this in your own experimental setup, as stability data for pre-diluted solutions is not available.
Sign In to View Organizational & Contract Pricing.
About This Item
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
Skip To
Product Name
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
biological source
bovine lung
form
DMSO solution
solubility
water: soluble
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| biological source bovine lung | biological source - | biological source - | biological source synthetic |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.
Application
Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer
Provides protection against endogenous enzyme activity in cell and tissue extracts
Provides protection against endogenous enzyme activity in cell and tissue extracts
Biochem/physiol Actions
Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.
Features and Benefits
Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases
Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C
Consistent performance: ready-to-use solution
Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C
Consistent performance: ready-to-use solution
Preparation Note
One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.
Other Notes
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Disclaimer
This cocktail should be stored at -20 °C.
related product
Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Signaling through extracellular signal-regulated kinase is required for spermatogonial proliferative response to stem cell factor.
Dolci S et al
The Journal of Biological Chemistry, 276(43), 40225-40233 (2001)
Acceleration and persistence of neurofibrillary pathology in a mouse model of tauopathy following anesthesia.
Planel E et al
Faseb Journal, 23(8), 2595-2604 (2009)
Interaction of Huntington disease protein with transcriptional activator Sp1.
Li SH et al
Molecular and Cellular Biology, 22(5), 1277-1287 (2002)
Megan D Schertzer et al.
Molecular cell, 75(3), 523-537 (2019-07-02)
Long noncoding RNAs (lncRNAs) cause Polycomb repressive complexes (PRCs) to spread over broad regions of the mammalian genome. We report that in mouse trophoblast stem cells, the Airn and Kcnq1ot1 lncRNAs induce PRC-dependent chromatin modifications over multi-megabase domains. Throughout the
A transgenic rat that develops Alzheimer's disease-like amyloid pathology, deficits in synaptic plasticity and cognitive impairment.
Liu L et al
Neurobiology of Disease, 31(1), 46-57 (2008)
Related Content
Research evaluating immune checkpoints may help identify those likely to benefit from PD-1/PD-L1 immunotherapy and reveal new immunotherapy targets for future investigation. See how the MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 was used to multiplex immune checkpoint molecules for NSCLC research.
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8340-125UL | 04061838044037 |
| P8340-1ML | 04061838256249 |
| P8340-2.5ML | 04061838249715 |
| P8340-5ML | 04061838256256 |
| P8340-.05ML | 04061837258572 |
-
What is the working concentration if adding to the whole saliva? Can I dilute it in water, store and then add to saliva?
1 answer-
Helpful?
-
-
Can I add the the protease inhibitor to RIPA as 100ul of inhibitor and 10ml RIPA buffer, aliquots and store them at -20C ?
1 answer -
Is this product suitable for downstream ChIP experiments?
1 answer-
Common protease inhibitors used in chromatin immunoprecipitation assays include PMSF, EDTA, Leupeptin, Aprotinin, and Pepstatin A. This product contains multiple protease inhibitors to provide broad-spectrum inhibition, making it suitable for downstream ChIP experiments.
Helpful?
-
-
Will the protease inhibitors in this product remain stable/functional through repeated freeze/thaw cycles? If not, is there a recommended limit to the number of freeze/thaw cycles? Should this product be aliquoted?
1 answer-
We have not studied P8340 product for stability in repeated freeze/thaw cycles. Internally, we have made a 25 mg/mL stock solution; by dissolving 500 mg in 20 ml of water; this stock is around 100 mM (100x); We then aliquot 1mL and freeze once at -20C.
Helpful?
-
-
Can this product be used for IL-6 detection using ELISA in rat tissue Homogenate? Can it be used along with PMSF without creating any problem in the assay?
1 answer-
This product can be used in rat tissue homogenate. It already contains serine and cysteine protease inhibitors. Note that PMSF has a short half-life in aqueous solutions, specifically 55 minutes at 25°C and pH 7.5.
Helpful?
-
-
How long is the protease inhibitor cocktail stable at room temperature?
1 answer-
This product ships without wet or dry ice and is stable for up to two weeks at ambient temperatures. The long-term stability of this material at room temperature has not been determined. The recommended long-term storage temperature is -20°C.
Helpful?
-
-
Can we dilute it in water? The experiment I plan to do will require 100ul of Protease inhibitor per vial, and I have more than 50 samples to run.
1 answer-
This product is ready to use and should be diluted into the lysis buffer.
Helpful?
-
-
Is the protease cocktail P8340 supplied as a 100X solution or a 1X solution?
1 answer-
This cocktail is essentially a 100X preparation. One milliliter of this solution is sufficient for the inhibition of endogenous enzymes found in 100 mL of lysate. Please see the link below to review this and additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/107/629/p8340dat-ms.pdfHelpful?
-
-
What is the stock concentration of the Protease Inhibitor Cocktail P8340? Is it 1x or 100x?
1 answer-
The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.
Helpful?
-
-
Do we need to add PMSF as well along with this cocktail? I intend to use this for RIPA buffer
1 answer-
PMSF is a serine protease inhibitor. You can certainly add along with the cocktail if needed.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service