S8820
Protease Inhibitor Cocktail
SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use
Synonym(s):
Protease Inhibitor Tablets, protease inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
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product name
SIGMAFAST™ Protease Inhibitor Tablets, For General Use
biological source
bovine lung
bovine milk
form
tablet
solubility
water: 1 tablet/100mL, clear to hazy, colorless
Storage temp.
2-8°C
Related Categories
General description
SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.
Specificity
Inhibits serine, cysteine, aspartic, and metalloproteases.
Application
SIGMAFAST Protease Inhibitor Tablets has been used:
- to wash raw semen for membrane protein analysis
- in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
- as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
- as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)
These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.
Features and Benefits
- Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
- Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
- Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
- Tested and optimized for general use: proven performance and reliability for a wide range of applications.
Components
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Quantity
One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
Physical form
Tablet that is water soluble.
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Danger
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Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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