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M1028

Magnesium chloride solution

Name: Magnesium chloride solution
Brand: SIGMA

Molecular Biology, 1.00 M±0.01 M

Sinónimos:

MgCl₂, dichloromagnesium, magnesium chloride

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Fórmula lineal:

MgCl₂

Número CAS:

7786-30-3

Peso molecular:

95.21

NACRES:

NA.52

PubChem Substance ID:

24896582

UNSPSC Code:

12352302

MDL number:

MFCD00011106

Grade:

Molecular Biology

Form:

liquid

Solubility:

water: soluble

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grade

Molecular Biology

Quality Level

300

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

technique(s)

DNA sequencing: suitable

impurities

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

solubility

water: soluble

density

1.080 g/cm³

cation traces

heavy metals (as Pb): ≤5 ppm

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Este artículo	68475	208337	449164
Sigma-Aldrich M1028 Magnesium chloride solution	Sigma-Aldrich 68475 Magnesium chloride solution	Sigma-Aldrich 208337 Magnesium chloride	Sigma-Aldrich 449164 Magnesium chloride
form liquid	form liquid	form powder	form -
solubility water: soluble	solubility -	solubility -	solubility H₂O: slightly soluble(lit.)
Quality Level 300	Quality Level 200	Quality Level 200	Quality Level 200
grade for molecular biology	grade for molecular biology	grade -	grade -
density 1.080 g/cm³	density -	density 2.32 g/mL at 25 °C (lit.)	density 2.32 g/mL at 25 °C (lit.)
impurities DNAse, none detected, Protease, none detected, NICKase, none detected, RNAse, none detected	impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)	impurities <5%	impurities ≤1500.0 ppm Trace Metal Analysis

General description

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -

to prepare buffer to lyse cells for immunoprecipitation[1]
as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.[2]
in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.[3]
It has been used to protect the liver from oxidative damage.
to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.[4]

Features and Benefits

Available as a Ready-to-use solution which reduces the need for preparation time.
Heavy metals (as Pb): ≤5 ppm
Free from DNase, RNase, NICKase and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

comparable product

Referencia del producto

Descripción

Precios

63069

Magnesium chloride solution, BioUltra, Molecular Biology, ~1 M in H₂O

63036

Magnesium chloride solution, BioUltra, Molecular Biology, ~0.025 M in H₂O

68475

Magnesium chloride solution, BioUltra, Molecular Biology, 2 M in H₂O

00457

Magnesium chloride solution, 0.1 M

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

ppe

Eyeshields, Gloves

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Protective Effects of Magnesium Chloride on Liver Enzymes and Biomarkers of Oxidative Stress in high fat diet fed Rats

Mohammed et, al

Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)

Osteocyte differentiation and the formation of an interconnected cellular network in vitro.

M J Mc Garrigle et al.

European cells & materials, 31, 323-340 (2016-05-25)

Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ECM environment, akin to that existing in vivo. In this study

Insulin-like growth factor-1 lowers spreading depression susceptibility and reduces oxidative stress.

Yelena Y Grinberg et al.

Journal of neurochemistry, 122(1), 221-229 (2012-04-25)

Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure

Analysis of androgen receptor rapid actions in cellular signaling pathways: receptor/Src association.

Antimo Migliaccio et al.

Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)

Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells

A chronic high-fat diet exacerbates contractile dysfunction with impaired intracellular Ca2+ release capacity in the skeletal muscle of aged mice.

Hiroaki Eshima et al.

Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1153-1162 (2020-03-28)

Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca2+ release in

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Protocolos

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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Product Information Sheet - M1028

Product Information Sheet

Número de artículo de comercio global

SKU	GTIN
M1028-10X1ML	04061834037774
M1028-100ML	04061838258427

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